protein synthesis inhibitor chx  (MedChemExpress)

Journal: Virology Journal

Article Title: Lamin A/C promotes HBV transcription by modulating histone modification associated with cccDNA minichromosome in an HBx-dependent manner

doi: 10.1186/s12985-025-02820-9

Figure Lengend Snippet: Lamin A/C enhances the stability of HBx. A-C HBx-3 × Flag and Lamin A/C overexpression plasmids or si-LMNA were co-transfected into HuH-7 cells. HBx-3 × Flag and Lamin A/C overexpression plasmids or siRNAs were co-transfected into HuH-7 cells at 72 h. A HBx protein levels were determined using western blotting with anti-Flag antibodies. The fold change in the densitometric ratio was normalized to that of GAPDH and then compared to that of the control; the data are shown in the right panel. B HBx mRNA was analyzed and quantified by qRT-PCR. C Cells were treated with cycloheximide (CHX) (10 µg/ml) at the indicated times, after which western blot experiments were performed to measure the protein expression of HBx. For ( A-B ), the means ± SDs of three independent experiments are shown. * P < 0.05

Article Snippet: The protein synthesis inhibitors cycloheximide (CHX) (66–81-9) and actinomycin D (HY-D1020) were purchased from MCE (New Jersey, USA).

Techniques: Over Expression, Transfection, Western Blot, Control, Quantitative RT-PCR, Expressing